Telomere length measurement by qPCR in birds is affected by storage method of blood samples

first_imgGiven the potential role of telomeres as biomarkers of individual health and ageing, there is an increasing interest in studying telomere dynamics in a wider range of taxa in the fields of ecology and evolutionary biology. Measuring telomere length across the lifespan in wild animal systems is essential for testing these hypotheses, and may be aided by archived blood samples collected as part of longitudinal field studies. However, sample collection, storage, and DNA extraction methods may influence telomere length measurement, and it may, therefore, be difficult to balance consistency in sampling protocol with making the most of available samples. We used two complementary approaches to examine the impacts of sample storage method on measurements of relative telomere length (RTL) by qPCR, particularly focusing on FTA (Flinders Technology Associates) cards as a long-term storage solution. We used blood samples from wandering albatrosses collected over 14 years and stored in three different ways (n = 179), and also blood samples from captive zebra finches (n = 30) that were each stored using three different methods. Sample storage method influenced RTL in both studies, and samples on FTA cards had significantly shorter RTL measurements. There was no significant correlation between RTL measured in zebra finch blood on FTA cards and the same samples stored either as frozen whole blood or as extracted DNA. These results highlight the importance of consistency of sampling protocol, particularly in the context of long-term field studies, and suggest that FTA cards should not be used as a long-term storage solution to measure RTL without validation.last_img read more

Ex-NFL player killed in parking dispute with neighbor, police say

first_img Written by FacebookTwitterLinkedInEmailUniversity of Colorado Athletics(AURORA, Colo.) — A former NFL player was fatally shot over the weekend in a dispute with a neighbor over a parking space, according to police.Anthony “T.J.” Cunningham, a former defensive back for the Seattle Seahawks and an assistant principal at a high school in Aurora, Colorado, was allegedly shot by a neighbor on Sunday and taken to a local hospital, where he died the following day, according to the Arapahoe County Sheriff’s Office.Police charged his neighbor, 31-year-old Marcus Johnson, with first-degree murder on Monday, accusing him of shooting Cunningham multiple times in the head and chest.Witnesses said the two neighbors met at a high school in Aurora, located about 10 miles east of Denver, to settle an ongoing parking dispute, according to a probable cause affidavit.Cunningham’s brother told police he accompanied the former player to the school’s parking lot, where the two planned to “box it out,” according to the affidavit.The two men were walking towards each other when Johnson allegedly shot him three times, striking him in the head and chest, according to the document.Johnson called 911 to report the shooting and police said they found a handgun in his vehicle. Johnson said Cunningham was armed with a bottle when he pulled the trigger, but Cunningham’s brother said he didn’t have a weapon, according to the affidavit.Johnson was originally booked on attempted murder, but the charge was amended after the victim’s death. He was being held without bail and it’s unclear if he has retained an attorney.Cunningham, 46, played for the Seahawks during the 1996 season until injuries ended his NFL career, according to the team’s website. He also played wide receiver and defensive back at the University of Colorado.The university’s football team said he was a “tremendous part of the CU community” who “touched countless others” during his time there, according to a statement.“We were deeply shocked and saddened to learn of the death of T.J. Cunningham,” the Colorado Buffaloes said in a tweet on Monday. “He was a good family man and had a strong passion for working with young people.”Copyright © 2019, ABC Radio. All rights reserved. February 20, 2019 /Sports News – National Ex-NFL player killed in parking dispute with neighbor, police saycenter_img Beau Lundlast_img read more

Naomi Osaka thanked by families of Trayvon Martin, Ahmaud Arbery at US Open

first_imgSeptember 9, 2020 /Sports News – National Naomi Osaka thanked by families of Trayvon Martin, Ahmaud Arbery at US Open FacebookTwitterLinkedInEmailScott Clarke / ESPN ImagesBy KATIE KINDELAN, ABC News(NEW YORK) — Tennis star Naomi Osaka received an unexpected and emotional thank you Tuesday night from the families of some of the victims of police brutality and racial injustice whose legacies she is highlighting at the U.S. Open.Osaka was surprised after her quarterfinal win with video messages from the mother of Trayvon Martin and the father of Ahmaud Arbery thanking her for honoring them during the tournament.“God bless you for what you’re doing and you supporting our family with my son,” Arbery’s father, Marcus Arbery, Sr., said in a video message played for Osaka on ESPN. “My family really, really appreciates that, and God bless you.”“I just want to say thank you to Naomi Osaka for representing Trayvon Martin on your customized mask and also for Ahmaud Arbery and Breonna Taylor,” said Sybrina Fulton, Trayvon’s mother, in a separate video message. “We thank you from the bottom of our hearts. Continue to do well. Continue to kick butt at the U.S. Open.”At each of her matches at the Open, Osaka, 22, has worn a different face mask featuring the name of a person who has been a victim of racial injustice. On Tuesday, Osaka’s face mask honored George Floyd, who died on May 25 after a Minneapolis police officer pinned him down and put his knee on his neck.Osaka appeared visibly moved while watching the messages from Fulton and Arbery, Sr., and tweeted later that she cried while watching their messages again.“I often wonder if what I’m doing is resonating and reaching as many people as I hope,” she wrote. “That being said, I tried to hold it in on set but after watching these back I cried so much. The strength and the character both of these parents have is beyond me. Love you both, thank you.”After Osaka wore her mask featuring Trayvon’s name at her fourth-round match, she tweeted about the impact of his death. Trayvon, 17, was killed Feb. 26, 2012, in Florida while walking home from a convenience store after neighborhood watch volunteer George Zimmerman confronted and later shot him. Zimmerman was acquitted of second-degree murder and manslaughter in July 2013.“I remember Trayvon’s death clearly. I remember being a kid and just feeling scared.I know his death wasn’t the first but for me it was the one that opened my eyes to what was going on,” Osaka wrote on Twitter. “To see the same things happening over and over still is sad. Things have to change.”Osaka joined fellow athletes in protest last month after the police shooting of Jacob Blake, a 29-year-old Black man who was shot seven times by police in front of his children. The shooting sparked civil unrest in Blake’s hometown of Kenosha, Wisconsin, and across the country.Osaka protested the shooting by not competing in an Aug. 27 scheduled semifinal match at the 2020 Western & Southern Open.Osaka has said that she brought seven face masks with her to the U.S. Open, one for each match if she makes it through to the finals.Copyright © 2020, ABC Audio. All rights reserved. Written bycenter_img Beau Lundlast_img read more

A Question of Property: Liz Brown, MD of Connells, Midlands

first_imgHome » News » A Question of Property: Liz Brown, MD of Connells, Midlands previous nextA Question of Property: Liz Brown, MD of Connells, MidlandsNigel Lewis talks to Liz about online agents and how the region’s housing market is in a world of its own, including new homes sales up by over 30% year-on-year.27th November 201701,495 Views How big is your part of Connells?I look after 55 branches and approximately 140 staff in the Midlands for Connells including land, new homes, mortgages services, sales and lettings.Are online/hybrid agents making headway?They were during the first six months of the year when the market was more buoyant, and the instruction-to sale conversion rate was high up at the 80%. It’s then that we saw a considerable take-up among vendors for hybrid agents.But as the market has become tougher, so this has changed. Vendors are realising the hard way that in more difficult markets, you need a little more expertise and proactive support to achieve a sale. You’ve got to know your customer base much better and be able to be able to put chains together.We are picking up instructions from online agents, which fall into two categories. These are either failed online attempts to sell – people who’ve tried online and haven’t sold and who are then seriously annoyed when they have to pay twice.Then there are the people who do get an offer via a hybrid-type agency listing, but the sale goes nowhere, and they have to abandon the offer and start again with us.Overall is the Midlands property market thriving or diving?On the one hand we’ve got a thriving economy with good employment and salaries, particularly in and around Birmingham which is a real hot bed at the moment, as well as the region’s other big cities. But on the other hand, homes remain affordable although prices aren’t as low as other parts of England north of us.The Midlands housing market is an interesting one because on the one hand we’re isolated from both the ups and down of the South, but also the transaction and price lows of the North.This year has been particularly good – resales are currently 18% up on the same period last year while new homes are doing even better and have increased by a third or more compared to last year.Sounds like an agent’s dream?It’s not all a bed of roses – some of the problems you see in the south including homes taking longer to sell and transactions beginning to drop off have been spreading to the Midlands in recent months. But some of that is seasonal too.And Connells in the Midlands is preparing now for the tenant fees ban next year too – we’re not sticking our heads in the sand and hoping it doesn’t happen. We need to be ready.I still think the ban and the other changes to the buy-to-let market aren’t a good idea – we’ve seen landlords starting to exit the market in the Midlands and stock is becomes shorter so rents are going to increase.The trouble is, the ban isn’t going to help the  people it was designed to help. They’ll be paying fewer fees to move, but rents will rise. It does seem very silly.We have a good rental sector here – properties rent quickly, although people are staying in privately rented homes for longer – of up to three years.hybrid agents Liz Brown online agents Birmingham connells West Midlands November 27, 2017Nigel LewisWhat’s your opinion? Cancel replyYou must be logged in to post a comment.Please note: This is a site for professional discussion. Comments will carry your full name and company.This site uses Akismet to reduce spam. Learn how your comment data is processed.Related articles BREAKING: Evictions paperwork must now include ‘breathing space’ scheme details30th April 2021 City dwellers most satisfied with where they live30th April 2021 Hong Kong remains most expensive city to rent with London in 4th place30th April 2021last_img read more

O.C. Spring Event Lineup Announced

first_imgThe popular Easter Stroll returns April 3. (Photo from 2019) Ocean City is planning to open the 2021 season with a number of safe events for families.“We’re looking forward to the return of as many events as possible this year, but health and safety will always be our top priority,” Mayor Jay Gillian said.He continued, “We may not be able to hold all of them, and some may look different. Every event that happens will be in full compliance with current public health guidelines and require masks and social distancing.”The city plans to make an announcement soon about the Spring Block Party, which is tentatively scheduled for May 1.Look for further announcements on events throughout the year.The Great Egg Hunt (March 27): The traditional beach event moves up onto the Boardwalk this year. Participating merchants will invite children up to age 7 to visit stores with their families to collect eggs filled with treats and surprises. The event will run from 1 p.m. to 4 p.m. or while supplies last. Flyers will direct families to participating stores.The Great Egg Hunt (April 3): Boardwalk merchants will provide children with a second opportunity to participate in the Egg Hunt from 1 p.m. to 4 p.m. Call 1-800-BEACH-NJ for more information.Easter Sunrise Service (April 4): A traditional non-denominational service by the sea begins at 6:30 a.m. at the Ocean City Music Pier (Moorlyn Terrace and Boardwalk). Call 609-399-6111 for more information.Dueling Pianos (April 4): The Philly Keys will perform their hit dueling pianos show starting at noon in front of the Ocean City Music Pier. The outdoor show provides free fun for the entire family. The Philly Keys will take a break for the Easter Fashion Stroll and then resume.Easter Fashion Stroll (April 4): Come dressed in your Easter’s best for our Fashion Stroll in front of the Music Pier. Judging begins at 1 p.m. Prizes awarded for Best Dressed Children, Teen, Adults and Family. For more information, call 609-399-6111.OC-Con Comic Book and Memorabilia Festival is a zany event. (Photo courtesy City of Ocean City)Comic Book and Memorabilia Show: (April 10): Shop comic book vendors from all genres 10 a.m. to 4 p.m. at the Ocean City Music Pier. This modified event will be vendor-specific. It will not include the Cosplay, discussion panels, celebrity appearances or Super Hero Runs of past OC-Con events. Capacity limits will be in place. Call 609-399-6111 for more information or to sign up as a vendor. Vendors can register online at under “Events.”Girls Weekend (April 10 and 11): Girls Weekend takes place on downtown Asbury Avenue between 6th Street and 14th Street. Shopping and dining specials 9 a.m. to 5 p.m. all weekend, accommodation packages, wellness classes, and so much more. For more information, call 1-800-BEACH-NJ. Doo Dah Parade (Canceled): Because outdoor gatherings larger than 50 people are still prohibited by executive orders from the governor, the April 17 Doo Dah Parade will be canceled. The city had been holding out hope that restrictions on outdoor events would be relaxed by this time, but the parade requires advance planning on the part of both the city and participants. The Mr. Mature America Pageant, which had been scheduled for that evening, will move to September with Barry Williams (who starred in “The Brady Bunch”) still on tap to be the host.Spring Beach Sweeps (Canceled): Due to the popularity of Ocean City’s spring beach cleanup event and the 50-person limit on outdoor gatherings, Ocean City will not participate in the Clean Ocean Action Spring Beach Sweeps this spring. Volunteers can visit to see other locations in the area that are participating, read safety guidelines and register.Sports Memorabilia Show (April 24): Shop sports vendors from all teams 10 a.m. to 4 p.m. at the Ocean City Music Pier. This modified event will be vendor-specific. Capacity limits will be in place. Call 609-399-6111 for more information or to sign up as a vendor. Vendors can register online at under “Events.”Free Paper-Shredding Day (May 1): Ocean City will hold its semi-annual Paper Shredding Day 8 a.m. to 3 p.m. at the Shelter Road Recycling Center off Tennessee Avenue. This no-contact event will observe all public health guidelines. The event is free to residents and property owners in Ocean City. Please bring a driver’s license or other proof of residency/home ownership.Gina Secrest, of Ocean City, gets a little help from Two Twin Crows owner, Mary Janasky, with some items at her booth in Stainton’s during the 2019 Girls Weekend.last_img read more

In Brief

first_img– Jenny Hudson, a former pastry chef with Claridges, will be making a cake for the first wedding anniversary of Prince Charles and his wife Camilla, Duchess of Cornwall. It will be a chocolate cake decorated with berries and chocolate swirls. Ms Hudson runs her Warwickshire based company, Sweet As, as an on-line operation ( She started the business with assistance from The Prince’s Trust and plans to open a shop in the near future.- Police are investigating a recent robbery at Kent Bakery shop Mr Pastry. A man wearing a balaclava entered the shop in Ramsgate, just before 8am. He pointed what appeared to be a children’s toy pistol at the woman behind the counter and demanded money. She handed over a small amount of cash.last_img

Thorntons plays it cool with bakery and desserts

first_imgThorntons has made the move into the branded chilled bakery and desserts sector with the launch of a nine-strong range into the grocery channel this month.Alongside the dessert range, manufactured for Thorntons by Elisabeth the Chef, the confectionery firm, which already has a successful ambient cakes and bites range, has introduced a range of four cookies, produced by Northumbrian Fine Foods. The firm said it was pitching the range above Cadbury’s and Nestlé’s offering in this category, but below premium ranges such as Gü, and hoped to capitalise on the “middle market”.Thorntons’ sales and operations director Barry Bloomer said that, with the firm’s turnover in the grocery retail division now at £53m and growing at over 40%, it was a “natural progression” to branch out into chilled desserts.The dessert range features: Sticky Toffee Pudding; Chocolate & Toffee Tart; Sticky Toffee Tart; Sticky Double Chocolate Pudding; and Treacle Tart, and will be available in all multiples, independents and convenience stores but not in Thorntons’ own stores.The cookie range is listed in Morrisons and Thorntons stores, with some skus to launch in Asda and Sainsbury’s this month.last_img read more

Making a Tilly Mint

first_imgIf you’re after a case study of how to start up a successful cake business from nothing, then look no further than Truro-based Tilly Mint Bakery. Maxine Nelson started the business in 2009 with just £126 in her pocket and, within one year, the bakery was turning over £147,000, with no grants, loans or family help.It has been a rapid ascent for Nelson, the artistic director behind the bakery’s whimsical cupcakes and fantastical confectionery. Having left her life behind in France 18 months ago, boarding a flight back to Stansted airport with just her hand luggage and no idea of what she would do, Nelson had to think fast about her future.Up until 2003, she had been running a number of businesses in the UK, including a café and chocolate shop in Brighton, beauty salons, sunbed shops, as well as working as a consultant to the cosmetic ingredients industry. A family decision saw her up sticks and move to France for a slower-paced rural life. But in 2004 she was diagnosed with cancer and underwent major surgery, developing brittle bones as a side-effect of her treatment. However, she refused to let things stop her and, once on the mend, she began to run a small café.After going through such a tumultuous time, Nelson began to take stock and started to re-evaluate what she wanted out of life. In 2007, she sold the café and the house was put on the market. Four days before completion the buyers pulled out.She said: “This sent me into a downward spiral. Even though I had kept on doing consultancy work for cosmetic ingredients, having no business and no house sale made me pretty depressed. On the spur of the moment in 2009, I booked a flight back to the UK.”It really was a single-minded decision, but I knew my husband and I wanted different things and my teenage daughter was happy to follow me at a later date, so I just needed to start doing something for myself.”Belgian inspirationAfter making her mind up in Stansted Airport to “give Cornwall a go”, she moved there with the intention of baking cupcakes. “I had started my career as a nurse, but soon moved over to the cosmetic ingredients industry, which involved lots of travel, including many trips to Belgium, where I fell in love with the patisserie and chocolate designs I saw, and where I took chocolate and patisserie courses,” she explained. “I was fascinated by the intricate and innovative creations I had seen. Sitting in that airport lounge with no plan really made me home in on what I could do and where I could go, and quickly.”By staying with a friend, Nelson managed to save up enough money to make cupcakes for Falmouth Week in 2009, and the Cornwall Pride event in Truro, where 500 quirky cupcakes were snapped up.This success enabled her to open a stall in Pannier Market in the city. “We used wallpaper tables covered in pretty paper as our display stands at first, but things happened very quickly and we moved into our current café space next to the bus station in March 2010,” she said.Nelson now has a separate bakery where she conjures up her cupcake creations, with the assistance of Mike Williams, 26, who literally got on his bike and cycled from the Midlands to work with her.She runs the café with the help of Clare Pope, 22, from Truro, and a small team of helpers. She said: “Clare has been a huge support, in the beginning only taking wages as and when I could pay her, and I could not imagine running the business without her.”The café, along with being a showcase for the cupcakes, also sells soups, sandwiches and crêpes, but Nelson, not being one to sit still, is now looking to wholesale for the bakery trade too, while taking the cupcakes to a new level.She has since worked with a number of business support organisations in the county, such as Partner to Succeed and Cornwall Enterprising Women (CEW), taking part in CEW’s Growth Programme, delivered in partnership with Truro and Penwith College, which aims to develop ambitious female entrepreneurs’ companies.She said: “Life is sweet and I intend for it to get sweeter still.”l Visit www.tillymintbakery.comlast_img read more

Trampled By Turtles Ends Hiatus With Album & Tour Announcement, New Single [Listen]

first_imgRoughly 16 months after closing their “first chapter,” Minnesota-based string band Trampled By Turtles have announced their return to the studio and the stage. The five-piece outfit will get back in the groove on May 4th when they release their new album Life Is Good On The Open Road and kick off a two-night  run at the Palace Theatre in St. Paul, MN.Of course, the band’s comeback tour won’t end with a pair of Minnesota dates. The group will hit the open road for a two-leg tour through Midwest, Northeast, Mid-Atlantic and West Coast, including two-night runs in Washington, DC and Chicago. Along the way, Trampled by Turtles will be joined by supporting acts Deer Tick, Hiss Golden Messenger, The Pines, Frankie Lee, The Oh Hellos, Dead Horses, Actual Wolf, and The Lil Smokies.In addition to announcing their return, Trampled By Turtles have also released “Kelly’s Bar,” the debut single for their forthcoming album. You can give the track a listen below. Ticketing information for the tour is available via their website.“Kelly’s Bar” Trampled By Turtles Tour DatesMay 4 St. Paul, MN – The Palace Theatre*May 5 St. Paul, MN – The Palace Theatre ^May 6 Madison, WI – Capitol Theater #May 8 Boston, MA – House of Blues **May 9 Philadelphia, PA – Union Transfer **May 11 New York, NY – Playstation Theater **May 12 & 13 Washington, DC – 9:30 Club **May 15 Raleigh, NC – Ritz **May 17 Asheville, NC – Highland Brewing Company **May 18 Nashville, TN – Ryman Auditorium **May 19 & 20 Chicago, IL – Thalia Hall ^^June 21 Tahoe, NV – Montbleau %June 22 Oakland, CA – Fox Theatre %June 23 San Diego, CA – Humphreys Concerts By The Bay %June 27 Seattle, WA – Woodland Park Zoo %June 29 Portland, OR – Oregon Zoo Amphitheater %June 30 Vancouver, BC – Commodore Ballroom %July 19 Morrison, CO – Red Rocks Amphitheatre !July 21 Missoula, MT – KettleHouse Amphitheater $July 22 Bozeman, MT – Bridger Brewing $* – The Pines^ – Frankie Lee# – Dead Horses** – Hiss Golden Messenger^^ – Actual Wolf% – Deer Tick! – The Oh Hellos + Dead Horses$ – The Lil Smokieslast_img read more

HeadCount Teams With Parkland Students & March For Our Lives For Dead & Co Tour Participation Row

first_imgHeadCount has announced a new addition to the Participation Row activism village on Dead & Company’s summer tour, which kicks off today in Mansfield, Massachusetts. March For Our Lives, the movement and organization started by high school students who survived the mass shooting in Parkland, Florida, will have a booth at each Dead & Company show on the band’s upcoming summer tour. Deadheads visiting the booth will have a chance to meet alumni of Marjory Stoneman Douglas High School and take photos with messages encouraging people to vote.The collaboration started when Parkland students attended the Dead & Co. concert in Sunrise, Florida, and met the members of the band. Bob Weir encouraged the students to join forces with HeadCount in an impassioned speech about using this tragedy to affect real change. As he noted, “You kids are making quite a stir, and it’s focused. It’s not the kind of thing that anyone can argue with because you’re right.” He also made note to direct them toward HeadCount to help bolster their efforts. Watch Weir’s speech to the Parkland students below.Following their encounter with the Parkland kids at Dead & Company in Sunrise, HeadCount later conducted voter registration efforts at the March For Our Lives demonstrations around the country on March 24th, registering more than 5,000 people to vote just that day. In addition, student activist David Hogg worked with HeadCount to organize voter registration drives at more than 1,000 high schools in the last few weeks. Considering all the good that’s already been done by the Parkland Students’ and HeadCount’s efforts, making March For Our Lives a featured guest on Participation Row was a natural next step for Headcount.Explains HeadCount executive director Andy Bernstein, “We know Bobby and the whole band were really moved by the Parkland kids, and working with these kids to organize voter registration drives has been an amazing experience for HeadCount. So bringing them on Dead & Co tour seemed like a perfect way to tie this all together.”The Participation Row activism village, co-organized by HeadCount and the environmental group REVERB, has been part of every Dead & Co tour dating back to the band’s debut in Fall 2015. March for Our Lives will be joined at various stops on the tour by “Dead Family” non-profit organizations including Wavy Gravy’s SEVA, the Rainforest Action Network, and the Owsley Stanley Foundation.Fans who take actions with three non-profits at Participation Row will also have a chance to win a signed D’Angelico guitar and receive a numbered, limited edition VOTE pin. The pins, designed in the style of the famous “LOVE” sculpture but with a Steal Your Face skull as the “O,” were donated by the Dead-centric accounting firm Schulman Lobel. A charity auction at each tour stop will also raise funds for the participating organizations. To date, HeadCount’s Dead & Co. auctions have generated over $500,000 for over 40 different charities.For further information on HeadCount’s voter registration efforts, Participation Row, and more, head to their website. For more information on March for Our Lives, head here.last_img read more